Fig. 5.

(See next page) Comparative analysis of deposition of C components and expression of C regulators in a single middle ear biopsy sample from a girl aged 2 years and 7 months. The patient had suffered from chronic otitis media with effusion (OME) for 5 months. Immunostaining for the complement components iC3b/C3c (a), C3d (b), C9 (c) and vitronectin (VN) (d) showed deposition of iC3b/C3c on the epithelial cell surface and C3d and VN on the epithelial basement membrane. Note the negative staining for C9 on the epithelium and connective tissue. The membrane regulator membrane cofactor protein (MCP) (e) was seen in the hyperplastic epithelium. The staining for decay-accelerating factor (DAF) (f) was weak but got stronger deeper in the connective tissue. CD59 (g) was strongly expressed on the epithelial cells. (Mag.: a,b,g,h, × 460; c, × 150; d, × 210; e, × 300; f, × 630.)