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Killing of the Fas-sensitive targets (Jurkat and M-3) and Fas-resistant target (K-562) by CD4+ tumour-infiltrating lymphocyte (TIL) effectors kept in IL-2 at least 6 days after restimulation (IL-2 alone), or preactivated with anti-CD3 for 3 h. Anti-Fas (ZB-4) or EGTA were added to these CD3-stimulated cultures to determine the effect of these agents on tumour lysis.
