Fig. 2.

Sequencing of expanded beta variable (BV) CDR3 peaks. (a) Persistence of clones within the CD8⁺CD28⁻ subset. (b) Expanded peaks of the same length within the CD8⁺CD28⁺ and CD8⁺CD28⁻ subsets. CDR3 amino acid sequences have been deduced from the nucleotide sequences. The abscissae show CDR3 loop length. The ordinates indicate relative proportion of cellular material sharing the same CDR3 loop length (fluorescence intensity). (b) Sequences are reported only once where identical within both CD8⁺ subsets. In (a) donor 1 is represented in panels i and ii (BV12 and 15), donor 2 in panel iii (BV15). In (b) donor 2 is represented in panels i and ii (BV5.2 and 15), donor 4 in panels iii and iv (BV5.2 and 21), and donor 5 in panels v and vi (BV5.1 and 12).