Fig. 3.
IL-8 release by alveolar macrophages from four different sheep after exposure to Chlamydia psittaci. Cells (1 × 105/well) (macrophages) or 5 × 103 cells/well (ST-6) were seeded into 96-well plates and exposed to: (a) 1 × 104 inclusion-forming units (IFU) C. psittaci; (b) 1 × 103 IFU C. psittaci; (c) 1 × 104 IFU heat-treated C. psittaci; (d) 1 × 103 IFU heat-treated C. psittaci; or (e) medium alone. Heat treatment was performed at 65°C for 1 h. All cultures were performed in quadruplicate. Supernatants were harvested after 24 h (hatched), 48 h (□) and 72 h (▪) and individually assayed for the presence of ovine IL-8 by ELISA. Note that the ordinate values vary between sheep.