Fig. 2.

Time course of MCP-1 production by endothelial cells. Monolayers of endothelial cells were incubated for 60 min at 37°C with medium alone, with 5 × 10⁷Staphylococcus aureus strain 42D, with 1 × 10⁷S. aureus strain CAPD (a) or with 100 U/ml recombinant human IL-β (b), washed and treated with lysostaphin and cultured for different time periods. The release of MCP-1 into the culture medium was measured and plotted against time of culture. Values represent means ± s.e.m. of three experiments with endothelial cells from different donors.