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TNFB Nco1 polymorphism. The polymerase chain reaction (PCR) was used to amplify a 368-bp fragment of the of the TNFβ genomic sequence and the PCR product was digested directly with 1 U of NcoI restriction enzyme. Lane 1 shows a homozygous for the cleaved product produced bands at 133 and 235 bp (with incomplete digestion of the 368-bp band) representing the allele TNFB1, while the uncleaved 368-bp product in lane 3 represents the allele TNFB2. Lane 2 shows a heterozygote pattern.
