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. 1999 Sep;117(3):425–429. doi: 10.1046/j.1365-2249.1999.01005.x

Fig. 2.

Fig. 2

TNF-308 G to A substitution. Polymerase chain reaction (PCR) was used to amplify a 107-bp fragment of the of the TNFβ genomic sequence which was digested with 1 U of NcoI. The cleaved product produced bands at 87 and 20 bp representing the TNF-308 A allele, while the uncleaved 107-bp product represented the TNF-308 G allele. Lanes 1–4, homozygote 87/20 TNF-308 A allele; lane 5, heterozygote 107, 87/20; lanes 6 and 7, homozygote 87/20; lane 8, homozygote 107 band TNF-308 G allele; lanes 9–11, homozygote 87/20 TNF-308 A allele.