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EA-M-MBL were prepared by incubation (15 min, 37°C) of E-M-MBL with IgM and IgG haemolysin, respectively, in Mg-ethyleneglycol tetraacetic acid (EGTA), washed in Mg-EGTA, and tested for lectin pathway haemolysis in human serum-Mg-EGTA. A twofold enhancement (from 9 to 20 LH50 U/ml) was observed upon presensitization with IgM, while fourfold increase (from 9 to 40 LH50 U/ml) was observed upon presensitization with IgG.
