Fig. 3.

Expression of Fas and FasL in hepatocytes and infiltrating leucocytes in the tolerogenic rat orthotopic liver transplantation (OLT) (DA-PVG) model. (a) At day 14 when the most serious rejection was observed in this tolerogenic OLT (DA-PVG) model, there was dense cell infiltration and scattered focal necrosis of liver cells with mononuclear cells through the sinusoids (mag. × 120). (b) By 63 days when the rejection was overcome and tolerance was induced, the above histological appearance of liver grafts returned to normal (mag. × 120). (c) Fas was expressed strongly on infiltrating lymphocytes and weakly on hepatocytes at day 14 (mag. × 240). (d) Fas was expressed on both hepatocytes and endothelium at day 63 (mag. × 240). (e) FasL was expressed on both infiltrating lymphocytes and hepatocytes at day 14 (mag. × 240). (f) FasL was expressed on hepatocytes at day 63 (mag. × 240). (g) CD3⁺ cells were detected by a rhodamine-conjugated goat anti-mouse antibody and cells expressing FasL by a FITC-conjugated goat anti-rabbit antibody. Cells which appear yellow in colour (i.e. infiltrating lymphocytes) are positive for both CD3 and FasL marker (mag. × 240). (h) Single-positive cells expressing CD3 appeared red in colour by labelling rhodamine (mag. × 240). (i) FasL⁺ cells were detected by a FITC-conjugated goat anti-rabbit antibody and cells expressing ATP7B by a rhodamine-conjugated goat anti-mouse antibody. Cells which appear yellow in colour (i.e. hepatocytes) are positive for both FasL and ATP7B markers (mag. × 240). (j) Single-positive cells expressing ATP7B appeared red in colour by labelling rhodamine (mag. × 240).