Fig. 1.

Effects of methotrexate (MTX) on cytokine production by purified CD45RA (a) and CD45RO (b) T cells from the peripheral blood (PB) of healthy donors after 2 days. PB CD45RA and CD45RO T lymphocytes were isolated using immunomagnetic beads and stimulated with anti-CD3 in the presence of indicated amounts of MTX. Supernatants were collected after 48 h and assayed for cytokine concentration by specific ELISA. Data are expressed as percentage of cytokine concentration in the absence of MTX (100%) and represent mean values ± s.d. of three independent experiments. Significant changes compared with the MTX-free culture condition: *P < 0.05.