Fig. 3.

Effects of methotrexate (MTX) during restimulation on pro- and anti-inflammatory cytokine production by T cell receptor (TCR)-primed CD45RA and CD45RO T lymphocytes. CD4⁺ T cells were primed via the TCR/CD3 complex in the presence of IL-2 for 9 days in the absence of MTX. Cells were then restimulated with anti-CD3 plus IL-2 in the presence of MTX for an additional 2 days. Cytokine concentrations in cell-free supernatants were then determined by specific ELISA. Data are expressed as percentage of cytokine concentration in the absence of MTX (100%) and represent mean values ± s.d. of three independent experiments. Significant changes compared with the MTX-free culture condition: *P < 0.05.