Fig. 2.

Kinetics and dose effects of IL-10 and tumour necrosis factor-alpha (TNF-α) release triggered by cell walls (CW) from Eubacterium aerofaciens(Eu.a.) or Streptococcus pyogenes(S.p.). Lipopolysaccharide (LPS) was used for comparison. Upper panels: peripheral blood mononuclear cells (PBMC; 2 × 10⁶/ml) were stimulated with CW (10 μg/ml) or LPS (1 μg/ml) for 6 h, 24 h and 48 h at 37°C. Lower panels: cells were stimulated with various concentrations of bacterial CW or LPS (1 μg/ml) at 37°C for 24 h. Each dot represents a mean ± s.d. from two (upper panels) or three (lower panels) separate experiments. •, LPS; ▪, Eu.a. CW; ▴, S.p. CW.