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FACS analysis of anti-PR3 MoAbs on primed neutrophils. Neutrophils were isolated and primed with tumour necrosis factor-alpha (TNF-α) for 15 min. MoAbs to PR3 were added and detected with goat anti-mouse FITC. All anti-PR3 MoAbs bound to PR3 expressed by neutrophils; a selection is shown here. Neutrophils incubated with MoAbs 12.8, PR3G-3 and PR3G-6 are shown. As a negative control neutrophils were incubated first with an irrelevant IgG1 MoAb (blank).
