Fig. 4.

Immunohistochemistry (A,B,C) and in situ hybridization (D) for Fas ligand (FasL) in the EAAU iris. (A) Control. The expression of FasL in the iris is weak and soluble FasL is stained red (fast red) in the anterior chamber. Corneal endothelium is stained strongly for FasL. (B) Nineteen days after immunization. EAAU strongly induced FasL expression in the iris. The infiltrating lymphocytes including chamber cells express FasL in the active stage of EAAU. (C) Thirty days after immunization. The expression of FasL in the iris remains increased after the invading cells have completely disappeared. (D) The iris is thickened by the infiltrating lymphocytes 19 days after immunization. Both the iris and the infiltrating lymphocytes express mRNA of FasL at this stage. Counterstain with methyl green. (Original mag., × 200.)