Fig. 1.

Effect of anti-IL-4R MoAb on IL-4 levels in cultures of human memory and naive T cells. Resting memory (CD45RA⁻) peripheral blood T cells (CD3⁺ T cells in five experiments or CD4⁺ T cells in seven experiments) (a) and paired resting naive (CD45RO⁻) CD3⁺ or CD4⁺ T cells (b) were cultured with P815/CD80 cells (5 × 10⁵/ml) in the presence or absence of anti-IL-4R MoAb (2.5 μg/ml). Anti-CD3 MoAb (UCHT1) was added at a concentration of 2 μg/ml. Supernatants were collected after 72 h of culture and the IL-4 concentrations in the supernatants were determined by ELISA. The results (mean ± s.e.m.) of independent paired experiments with cells from 12 non-atopic adult donors are shown. NS, Not significant. ***P < 0.001.