Fig. 3.

Histology of pancreatic islets in MLD-SZ-induced diabetes in mice. (a) Massive mononuclear cell (MNC) infiltration (arrow) 10 days after disease induction. (H–E staining, mag. × 250.) (b) CD4⁺ cells (arrows) within MNC infiltrate 20 days after disease induction. (Immunoperoxidase staining, mag. × 250.) (c) α₁-antitrypsin (α₁-AT)⁺ cells at the periphery of MNC infiltrate 20 days after disease induction. (Mag. × 312.) (d) Extensive necrosis (arrow) and loss of islet margins 30 days after disease induction. (H–E staining, mag. × 312.) (e) Proliferation of connective tissue 84 days after disease induction. (Maldonado staining, mag. × 125.) Histology of pancreatic islets in A77 1726-treated diabetic mice (dose 25 mg/kg per day, given from day 10–19). (di) Well preserved islets without MNC infiltrate and necrosis 30 days after disease induction. (H–E staining, mag. × 250.) (ei) Absence of islet fibrosis 84 days after disease induction. (Maldonado staining, mag. × 125.)