Fig. 5.

Similar L chain variable regions encode thyroid peroxidase (TPO) binding Fab selected using native (N) or denatured (DN)-TPO. Derived amino acid sequences (partial) are shown for clones selected from: (i) the GA-phage display library panned on DN-TPO (DN clones); (ii) the same library panned on N-TPO (N clones); (iii) the GA-bacteriophage λ library using 125I-N-TPO, ‘G(N)’ clones. The clones are grouped according to the derived amino acid sequences of their H chain D regions (see Fig. 6). Included for comparison are the amino acid sequence of their closest germ-line genes, namely Vg; O12/O2 and A27 [26].