Fig. 2.

Immunofluorescent studies of glomerular lesions in injected SCID mice (× 300). (a,c,e) Glomeruli of mice injected with the B1 clone alone. (b,d,f) Glomeruli of mice injected with both the B1 and Sp6 clones. (a,b) IgG. (c,d) IgM. (e,f) Mac-2 staining. In the glomeruli of the mice injected with the combination of the B1 and Sp6 clones both IgG and IgM deposits are remarkable (b,d), and a number of Mac-2⁺ cells are observed (f), while in the case of the B1 clone alone, IgG was positive (a), but IgM was negative (c), and there was an absence of Mac-2⁺ cells (e). Glomeruli of the mice injected with the Sp6 clone alone were negative for all the staining reactions (not shown).