Fig. 3.

Levels of IFN-γ and IL-5 present in supernatants collected in the in vitro cell priming system (IV) assays. Non-adherent peripheral blood mononuclear cells (PBMC) from normal donors were co-cultured with autologous infected or uninfected macrophages for 7 days, and after this period they were restimulated for 48 h, again employing autologous infected macrophages. Supernatants were collected and cytokine levels determined by ELISAs. (a) Production of IFN-γ (pg/ml). (b) Production of IL-5 (pg/ml). Each triangle represents an individual donor. Concentrations of IFN-γ and IL-5 in cultures with uninfected macrophages have been subtracted. The levels of IFN-γ obtained from cultures with uninfected macrophages ranged between 0.0 and 270.0 pg/ml and the levels of IL-5 from the same cultures were between 0.0 and 5.5 pg/ml. There are no significant differences (P > 0.05) between the different groups infected with dhfr-ts⁻, CC1 or LV39 concerning the production of IFN-γ or IL-5.