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Northern blots showing the effects of different cytokines on PTX3 expression by osteoarthritis (OA) synoviocytes (a) and normal skin fibroblasts (b). (c) Induction of IL-8 mRNA in OA synoviocytes by the preparation of the IL-1β used in all the PTX3 experiments. Cytokine concentrations and times of stimulation are detailed in Patients and Methods. Expression of GAPDH mRNA is shown for normalization of the quantities of RNA loaded in gel lanes.
