Table 3.
Effect of IL-10 and stimulation by CD7, CD69, and LFA-1 on cell proliferation of isolated γδ T cells from tuberculosis (TB) and leprosy patients stimulated with Mycobacterium tuberculosis or M. leprae
| – | IL-10 | a-IL-10 | a-CD69 | a-CD7 | a-LFA-1 | x63 | |
|---|---|---|---|---|---|---|---|
| TB | 13·6 | 9·9 | 13·9 | 17·1 | 15·6 | 16·9 | 14·0 |
| PPD+ | 15·8 | 10·1 | 15·1 | 18·6 | 19·0 | 20·9 | 16·3 |
| TT | 8·6 | 5·9 | 9·7 | 14·7 | 15·8 | 14·6 | 9·0 |
| LL | 1·0 | 1·0 | 4·8 | 1·3 | 4·3 | 3·9 | 1·6 |
| Lep(+) | 10·4 | 6·6 | 11·0 | 15·9 | 16·9 | 19·9 | 10·9 |
Fresh isolated γδ T cells from patients and controls were cultured for 5 days in the presence of M. tuberculosis or M. leprae plus IL-10, or MoAbs against IL-10, CD69, CD7 or LFA-1; at the end of cell culture, lymphocyte proliferation was assessed by 3H-TdR incorporation, as described in Materials and methods. The irrelevant x63 MoAb was employed as an isotype-matched negative control.
TT, Tuberculoid leprosy patients; LL, lepromatous leprosy patients; Lep(+), healthy contacts lepromin+.
Data correspond to the stimulation index of a representative experiment out of five.