Fig. 1.

Cytokine expression of NK leukaemic cells of HMB-EBV-NK disease. (a) Purified NK cells from a patient with HMB-EBV-NK disease and a normal subject were cultured in the presence of rIL-2 (50 U/ml). The IL-4-, IL-5-, IL-10- and IFN-γ-producing capacity of each NK cell was examined by ELISPOT assay. Data are expressed as the mean ± s.e.m. of results in duplicate experiments. (b) Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed with specific primers for IL-4, IL-5, IL-10, IFN-γ, and β-actin in NK cells freshly isolated from the patient. Normal peripheral blood mononuclear cells activated with rIL-2 (50 U/ml) or concanavalin A (2 μg/ml) were used as control.