Table 2.

Removal of IgG1 antibodies decreases TNF-α secretion from PPD stimulated monocytes

* PPD (10μg/ml) stimulated monocytes (1 × 10⁶) in the absence of plasma resulted in secretion of 1760 pg/ml of TNF-α

† Patient plasmas were absorbed with protein ‘A’ to remove IgG1 antibodies.

‡ Stimulation in monocytes was assessed in the presence of protein ‘A’ treated plasma but without concurrent PPD stimulation. Monocytes were also stimulated with PPD in the presence of either unabsorbed or absorbed plasma at a dilution of 1:500.