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DNA fragmentation on cells obtained from Trypanosoma cruzi-infected mice. Spleen mononuclear cells (SMC) from normal 8-day, 15-day or 180-day-infected mice were cultured with medium alone or LPS (20 μ g/ml) during 6 h. DNA released into the supernatant by SMC was electrophoresed to monitor DNA fragmentation. Molecular size markers (Φλ DNA-EcoRI-Hind III digest) are shown in lane M. Data are representative of three independent experiments.
