Fig. 5.

Incorporation of fluorescein-labelled dUTP into DNA strand breaks of purified B cells from Trypanosoma cruzi-infected and normal mice. B cells from normal (a) or infected mice at day 15 after infection (b) were cultured during 6 h (2 × 10⁶ cells/well) in medium alone (left panels) or with LPS (right panels). The cells were then incubated with FITC-labelled dUTP and terminal deoxitransferase according to Promega Kit directions. The percentages of apoptotic B cells are indicated. Staining without the TdT enzyme during TUNEL reaction is shown by open histograms The data are representative of two independent experiments.