Fig. 7.

Cell cycle analysis of B220⁺ cells from Trypanosoma cruzi-infected and normal mice. Spleen mononuclear cells (SMC) from normal (a) or infected mice (b) were explanted at day 15 after infection and cultured during 6 h with medium alone (left panels) or with 20 μ g/ml LPS (right panels). Cells were double-stained with FITC-labelled anti-mouse B220 and propidium iodide. B220⁺ cells were gated and the percentages of B cells in G₀/G₁ (M1), S/G₂/M (M2) or sub G₀/G₁ (M3) cell cycle stages are indicated.