Table 2.
TCR Vα24 CD4−CD8− double-negative (DN) T cell population in peripheral blood lymphocytes (PBL) from patients with atopic diseases
| Materials | Vα24+ DN T cells /PBL (%) | Vβ11+α24+ DN T cells (%) | NKR-P1A+α24+ DN T cells (%) | NKR-P1A+ Vβ11+α24+ DN T cells (%) |
|---|---|---|---|---|
| Cont-1 | 0·24 | 47·2 | 45·2 | 45·8 |
| Cont-2 | 0·13 | 44·9 | 51·2 | 43·7 |
| Cont-3 | 0·17 | 51·0 | 61·7 | 49·4 |
| Cont-4 | 0·19 | 60·8 | 62·3 | 59·5 |
| Cont-5 | 0·21 | 83·3 | 81·4 | 80·1 |
| Mean (s.d.) | 0·19 (0·04) | 57·4 (15·6) | 60·4 (13·8) | 55·7 (14·9) |
| A-1 | 0·06 | 9·9 | 15·3 | 7·5 |
| A-2 | 0·14 | 12·3 | 11·2 | 8·6 |
| A-3 | 0·07 | 8·2 | 14·4 | 7·6 |
| A-4 | 0·08 | 7·3 | 11·2 | 6·2 |
| A-5 | 0·06 | 11·4 | 12·6 | 8·8 |
| A-6 | 0·11 | 7·7 | 9·2 | 6·5 |
| A-7 | 0·03 | 5·2 | 21·2 | 4·2 |
| A-8 | 0·03 | 8·1 | 10·0 | 6·1 |
| A-9 | 0·06 | 9·3 | 9·9 | 6·3 |
| Mean (s.d.) | 0·07 (0·03)* | 8·8 (2·1)* | 12·8 (3·7)* | 6·9 (1·4)* |
The expression of TCR Vβ11 and NKR-P1A in peripheral blood Vα24 DN T cells from healthy subjects (cont-1 to -5) and patients with atopic diseases (A-1 to -9) was analysed by FACS using anti-CD4 peridinin chlorophyll protein (PerCP) plus anti-CD8 PerCP, anti-Vα24 biotin + streptavidin- allophycocyanin (APC), anti-Vβ11 FITC, and NKR-P1A + anti-mIgGl PE. PBL, Peripheral blood lymphocytes.
*
Significantly different from the mean value of healthy subjects, P <0·001.