Table 3.
Frequencies of invariant Vα24Jα Q double-negative (DN) T cells in patients with atopic diseases
| Source | Vα24Jα Q/Vα24† | Vα24+ DN T cells‡ (/mm3) | Vα24Jα Q DN T cells (/mm3) |
|---|---|---|---|
| Cont-1 | 15/20 | 3·9 | 2·9 |
| Cont-2 | 16/20 | 2·3 | 1·8 |
| Cont-3 | 16/18 | 2·9 | 2·6 |
| Cont-4 | 17/19 | 3·4 | 3·0 |
| Cont-5 | 18/18 | 3·2 | 3·2 |
| Mean (s.d.) | 86·7% (9·6%) | 3·1 (0·6) | 2·7 (0·5) |
| A-1 | 2/17 | 1·0 | 0·11 |
| A-2 | 1/16 | 2·9 | 0·18 |
| A-3 | 0/16 | 1·2 | 0 |
| A-4 | 0/16 | 1·6 | 0 |
| A-5 | 0/16 | 1·4 | 0 |
| A-6 | 0/16 | 2·5 | 0 |
| A-7 | 3/16 | 0·4 | 0·05 |
| A-8 | 0/16 | 0·5 | 0 |
| A-9 | 0/16 | 0·8 | 0 |
| Mean (s.d.) | 4·1% (6·3%)* | 1·4 (0·8)* | 0·04 (0·06)* |
*
TCR Vα24 cDNA clones were randomly isolated from the polymerase chain reaction (PCR)-amplified libraries of DN T cells from healthy subjects (cont-1 to -5) and patients with atopic diseases (A-1 to -9), and the frequency of Vα24J αQ rearrangement among Vα24 cDNA clones was determined by sequencing of complementarity determining region 3 (CDR3) of TCR Vα24 genes.
†
TCR Vα24 DN T cells in peripheral blood lymphocytes from healthy subjects and patients with atopic diseases were analysed by FACS.
*
Significantly different from the mean value of healthy subjects, P <0·001.