Table 4.

Vα24⁺ Vβ11⁺ double negative (DN) T cells from healthy subjects predominantly produce IFN-γ

	Th0 (%)	Th1 (%)	Th2 (%)
Cont-1	8·51	75·20	1·39
Cont-2	5·80	73·41	1·10
Cont-3	11·45	80·60	0·64
Cont-4	6·30	81·20	0·54
Cont-5	3·52	82·59	0·82
Mean (s.d.)	7·11 (3·00)	78·60 (4·72)	0·90 (0·34)

Sorted DN T cells from healthy subjects were stimulated with phorbol myristate acetate (PMA; 25 ng/ml) plus ionomycin (1 μm) for 4 h, and cytokine production was evaluated by intracellular staining for IL-4 and IFN-γ on Vα24 Vβ11 cells. Th0, IL-4⁺; IFN-γ⁺; Th1, IL-γ⁺ Th2, IL-4⁺ IFN-γ⁻.