Fig. 1.

The effect of auranofin (AF) on the cytotoxicity induced by 100 ng/ml of TNF-α. Human umbilical vein endothelial cells (HUVEC) were treated for 30 min with AF, rinsed and then exposed to TNF-α for 24 h; subsequently unstimulated polymorphonuclear neutrophils (PMN) were added. Mean and s.e.m. values for five experiments, run in duplicates. **P < 0001; *P < 0·01 compared with controls, i.e. cytotoxicity induced by TNF-α without AF. No cytotoxicity was found when HUVEC were treated with TNF-α and AF without any PMN present.