Fig. 3.

The effect of auranofin (AF) on TNF-α-induced NF-κ B activation. Human umbilical vein endothelial cells (HUVEC) were left untreated (lanes 1–3) or incubated with 1 μg/ml AF for 30 min (lanes 4 and 5). Cells were then stimulated with TNF-α (100 U/ml) for 2 h (lanes 2–5). Reactions were performed in the presence of a 100-fold molar excess of unlabelled NF-κ B consensus oligonucleotide (lanes 3 and 5). Nuclear extracts were analysed subsequently for DNA binding activity in electrophoretic mobility shift assays. The position of the inducible NF-κ B complexes is noted.