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Immunoblot of aminophenylboronic acid (APB)-purified proteins (5 μg/lane) from the immune complexes of patient and control representatives of Caucasian (C) or North American Indian (NAI) descent probed with anti-Nε(carboxymethyl)lysine (CML) MoAbs. Lane 1, C diabetic; lane 2, C RA, anti-advanced glycation end-product (AGE)-positive; lane 3, C RA anti-AGE−; lane 4, C normal; lane 5, NAI RA anti-AGE+ lane 6, NAI RA anti-AGE−; lane 7, NAI normal; lane 8, molecular weight markers.
