Table 2.
Condition (concentration of TNF-α) | Percentage of PMN expressing PR3 | P |
---|---|---|
Basal | 18·0 ± 3·5 | – |
TNF-α (1 ng/ml) | 25·3 ± 3·3 | 0·044* |
TNF-α (3 ng/ml) | 36·0 ± 0·8 | 0·014* |
TNF-α (10 ng/ml) | 52·3 ± 2·2 | 0·002* |
PMN were incubated with TNF- a in increasing concentrations ranging from 1 to 10 ng/ml for 10 min. Subsequently, PMN (1 × 106/ml) were incubated with MoAbs to PR3 (WGM2) for 20 min. After washing, second antibody (fluorescein (DTAF)-conjugated F(ab)2 goat anti-mouse (IgG) was added. Subsequently the labelled cells were fixed with PBS containing 1·5% paraformaldehyde. The surface expression of PR3 given as the percentage of PMN showing PR3 expression on flow cytometric analysis. Data are means ± s.e.m. from nine experiments using cells from nine different donors.
Statistically significant difference between stimulated (TNF-α) and unstimulated PMN (basal).