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Flow cytometric analysis of CD40L expression in the CD4+ T cells from young (□) and elderly (▪) subjects. CD4+ T cells were co-cultured with LCD32/CD80 for 60 h in the medium containing anti-CD3 (0·01 μg/ml). Induction of CD40L by this co-culture in each sample was assessed by measuring mean fluorescence intensity (MFI) of CD40L in flow cytometric analysis (a). Mean +s.e.m. of MFI in the young (□) and elderly (▪) groups are shown (b). **P < 0·05.
