Fig. 1.

Summary of two independent ELISAs with representative Fab-phage supernatants from the MM and MG libraries after the 4th round of panning on intravenous immunoglobulin preparations (IVIG). Coated proteins consisted of IVIG or Fc fragments in equal concentrations as well as PBS/casein blocking buffer to also detect non-specific binding. Negative controls for each ELISA were pComb3H-transfected Escherichia coli phage supernatant (pComb3H) and SB medium containing VCSM13 helper phage (SB + M13). Bound Fab-phages were detected with horseradish peroxidase-labelled anti-M13 antibodies.