Table 1.

IL-12 (pg/ml) production by polymorphonuclear neutrophils (PMN) exposed to acapsular (7698) or encapsulated (6995) Cryptococcus neoformans from normal subjects or HIV-infected patients

Subjects	CD4/mm3	RNA copies/ml	None	LPS (10 µg/ml)	MoAb 18B7	C. albicans	C. albicans + MoAb 18B7	7698	7698 + MoAb 18B7	6995	6995 + MoAb 18B7
Controls‡	1051 ± 75	0	12 ± 1	39 ± 4	13 ± 2	40 ± 5	32 ± 3	16 ± 2	14 ± 2	15 ± 2	14 ± 1
Pt no. 5	819	76 980	60	30	ND	21	18	11	10	16	15
Pt no. 1	815	89 843	21	20	18	7	9	11	9	9	10
Pt no. 6	600	8577	19	35	ND	ND	ND	22	ND	9	9
Pt no. 16	445	92 633	22	19	ND	17	ND	13	ND	20	11
Pt no. 3	384	12 764	25	15	18	10	9	12	13	12	14
Pt no. 15	384	45 678	22	128	20	22	ND	24	ND	14	15
Pt no. 8	160	830 400	80	15	ND	ND	ND	6	7	12	7
Pt no. 9	130	490 776	5	5	2	3	1	0	1	2	1
Pt no. 7	128	1401 144	65	12	ND	6	7	2	3	10	8
Pt no. 12	93	730 769	91	129	85	76	78	92	94	85	87
Pt no. 13	82	5388	74	80	ND	ND	ND	30	27	33	35
Pt no. 10	80	564 932	50	21	ND	ND	ND	12	10	9	12
Pt no. 14	26	1928 691	96	85	91	52	48	90	ND	82	84
Mean	318 ± 78	482·96	48 ± 9†	46 ± 12	39 ± 16	24 ± 8*	24 ± 11	25 ± 8**	19 ± 10	24 ± 7	24 ± 8***
± s.e.m.		± 169·07

PMN (4 × 10⁶) were exposed for 18 h to the indicated fungi at an E:T ratio of 1:2. Supernatants fluids were harvested and cytokine levels were determined by ELISA. ND, Not determined eight patients.

^*P < 0·024 (HIV-infected treated versus HIV-infected untreated cells), 13 patients

^**P < 0·022 (HIV-infected 7698-treated versus HIV-infected untreated cells), 13 patients

^***P < 0·022 (HIV-infected MoAb plus 6995 treated vs. HIV-infected untreated cells)

^†P < 0·014 (HIV-infected versus control cells).

^‡The results are the mean ± s.e.m. of experiments with cells from 13 different donors.