Fig. 2.

Immunoblotting of the affinity-purified tRNP^(Ser)Sec antigen from HeLa cell sonicate, with autoimmune hepatitis (AIH) sera. The immunopurified HeLa cell extract was fractionated on 10% polyacrylamide/SDS gels, blotted onto nitrocellulose, and probed with antisera as indicated. The positions of molecular weight markers (in kD) are shown on the right. Numbers at the top of each lane correspond to the number given to each patient, lanes DOD and DPP show the immunoblot reaction of anti-tRNP^(Ser)Sec reference sera, and lane Normal serum shows the immunoblot reaction of a serum from a healthy blood donor volunteer.