Fig. 5.
Influence of non-thiol angiotensin-converting enzyme (ACE) inhibitors on activation-induced cell death and IL-2 production in T cell hybridomas. (a) Cell viability. N3-6-71 cells were cultured in control 24-well plates or in anti-CD3 antibody-coated 24-well plates (5 × 105 cells/well), in the presence or absence of the indicated concentrations of N-succinyl-L-proline or lisinopril. After 50 μl of culture supernatant were removed from each well for the measurement of IL-2 at 24 h of cultivation, the cells were harvested and then cell viability was assessed by trypan blue dye exclusion. (b) IL-2 production. N3-6-71 cells were cultured in described above and concentrations of IL-2 in the culture supernatant were measured by ELISA. These results are expressed as the mean ± s.d. of triplicate cultures (a,b). ▪, Control; □, anti-CD3.