Age-related frequency of IFN-γ responses to all three variants of LSA-1 84–92. Peripheral blood mononuclear cells from a total of 117 individuals were incubated with individual peptides as described in SUBJECTS and METHODS. The number of persons in age group was the following: 2–3-year-olds, 16; 4-year-olds, 15; 5-year-olds, 12; mean 7-year-olds (range 6–10), 19; mean 15-year-olds (range 11–20), 13; mean 26-year-olds (range 21–30), 17; mean 33-year-olds (range 31–40), 18; mean 48-year-olds (range 41–54), 7. The trend line has r2 = 0.85.