Table 1.
Reactivity of 1H6.2, 45.30 and control MoAbs against different protein and protein–hapten antigens (ELISA)
| Antigen | 1H6.2* | 45.30 | S13.11 | SP6 |
|---|---|---|---|---|
| bLF-MBP | 1·950 | 0·545 | 0·106 | 0·153 |
| BSA | 0·150 | 0·030 | 0·113 | 0·020 |
| KLH | 0·030 | 0·027 | 0·428 | 0·450 |
| BSA–FITC | 0·022 | 0·036 | 0·123 | 0·206 |
| KLH–FITC | 0·017 | 0·039 | 0·332 | 2·0 |
| BSA–TNP | 0·265 | 0·020 | 0·174 | 2·0 |
| Ly | 0·027 | 0·033 | 0·073 | 0·046 |
| Tfn | 0·012 | 0·024 | 0·099 | 0·025 |
| OVA | 0·013 | 0·021 | 0·086 | 0·114 |
Data are expressed as mean optical density (OD) values of triplicates. For the sake of clarity s.d. values (s.d. < 15%) are not shown.
*
All MoAbs were used at a final concentration of 300 μg/ml. Reactivity of secondary goat anti-mouse antibodies was measured and subtracted from the data reported in the table.
bLF, Bovine lipid-free; BSA, bovine serum albumin; KLH, keyhole limpet haemocyanin; TNP, trinitrobenzene sulphonic acid; Ly, lysozyme; Tfn, human transferrin; OVA, ovalbumin.