Table 2.
Cell surface expression of MBP epitopes revealed by 1H6.2 and 45.30 MoAbs (cytofluorimetric indirect assays)
| Cells | Ctrl* | 1H6.2 | 45.30 | SP6 | S13.11 |
|---|---|---|---|---|---|
| Macrophages | 0·128 | 0·630 | 0·578 | 0·370 | |
| Endothelial | 0·412 | 0·472 | 0·414 | 0·394 | 0·405 |
| Fibroblasts | 0·522 | 0·544 | 0·525 | 0·534 | |
| DC | 0·165 | 0·227 | 0·225 | 0·218 | |
| PBMC | 0·134 | 0·139 | 0·145 | 0·130 | 0·147 |
| TEC | 0·290 | 0·255 | 0·191 | 0·242 | 0·190 |
| Thymocytes | 0·116 | 0·119 | 0·119 | 0·117 | |
| CFPAC-1 | 0·654 | 2·42 | 0·716 | 0·673 | |
| MOLT-4 | 0·402 | 0·414 | 0·402 | 0·441 | |
| B-EBV | 0·155 | 0·281 | 0·336 | 0·178 | |
| Raji | 0·187 | 0·212 | 0·210 | 0·186 |
Data are expressed as mean fluorescence intensity (MFI). MFI values of cells labelled with irrelevant SP6 and/or S13.11 IgM MoAb are also shown.
*
In this column MFI values of cells labelled with secondary antibody only are reported.
DC, Dendritic cells; PBMC, peripheral blood mononuclear cells; TEC, thymic epithelial cells.