Table 3.
Intracellular expression of MBP-epitopes revealed by 1H6.2 and 45.30 mAbs (Cytofluorimetric indirect assays)
| Cells | Ctrl* | 1H6.2 | 45.30 | SP6 | S13.11 |
|---|---|---|---|---|---|
| Macrophages | 0·705 | 5·55 | 1·05 | 0·814 | |
| Endothelial | 0·649 | 6·89 | 2·80 | 0·840 | 0·963 |
| Fibroblasts | 0·470 | 1·37 | 0·485 | 0·533 | |
| DC | 0·488 | 0·760 | 0·947 | 0·507 | |
| PBMC | 0·172 | 3·09 | 0·585 | 0·245 | 0·176 |
| TEC | 0·176 | 3·19 | 1·04 | 0·357 | |
| Thymocytes | 0·190 | 3·59 | 0·321 | 0·236 | |
| CFPAC-1 | 1·21 | 42·7 | 6·78 | 2·37 | |
| MOLT-4 | 1·26 | 7·02 | 1·66 | 2·05 | |
| B-EBV | 0·487 | 21·1 | 1·44 | 1·57 | |
| Raji | 0·526 | 3·00 | 0·750 | 0·501 |
Data are expressed as mean fluorescence intensity (MFI). MFI values of cells labelled with irrelevant SP6 and/or S13.11 IgM MoAb are also shown.
*
In this column MFI values of cells labelled with secondary antibody only are reported.
DC, Dendritic cells; PBMC, peripheral blood mononuclear cells; TEC, thymic epithelial cells.