Fig. 3.

Antibody idiotypes of anti-flavoprotein antibody (αFp-Ab) in the sera of coxsackievirus B3 (CVB3)-infected mice. Thirty mice were infected with CVB3. Sera of 10 mice each were collected 2, 7 and 9 weeks p.i. and tested on Western blots with 6-hydroxy-d-nicotine oxidase (6HDNO) as flavoprotein antigen with goat anti-mouse IgM-specific and goat anti-mouse IgG-specific second antibody. Sera of mice 2 weeks p.i. showed α6HDNO-Ab of the IgM isotype (a, 3, 4, 5, 6, 9, 10) and the IgG isotype (b, 2, 3, 6, 9). The sera of mice 7 weeks (b, 5) and 9 weeks p.i. (b, 6, 7, 9) were of the IgG isotype only. The black line on the Western blots served as orientation.