Fig. 5.

Sera of coxsackievirus B3 (CVB3)-infected mice recognize the endoprotease Lys-C generated 6-hydroxy-d-nicotine oxidase (6HDNO) FAD peptide but not the tryptic FAD peptides. 6HDNO was digested with endoprotease Lys-C (lanes 1 and 3) or trypsin (lanes 2 and 4), the peptides were separated by SDS–PAGE on a 12% or a 17·5% polyacrylamide gel, respectively, and blotted to nitrocellulose. The Western blots were decorated with the serum of the CVB3-infected mouse no. 8 (lanes 1 and 4) or with rabbit anti-6HDNO serum as control (lanes 2 and 5). Lanes 3 and 6 show the UV light fluorescence in 10% acidic acid of small molecular weight endoprotease Lys-C and tryptic peptides. The mouse serum reacted only with the endoprotease Lys-C-generated FAD peptide (lane 1) but not with the trypsin-generated FAD peptides (lane 4). The sizes in kD of molecular weight markers are indicated on the left and right sides of the panel.