Fig. 3.

Effect of the blockade of autocrine TGFβ signaling by DNRII expression on the activation of MAP kinases and the level of PTEN. (A) MCF-7 control (C) and DNRII cells were treated with or without tetracycline at 1 μg/ml for 3-4 days and harvested at exponential growth phase for Western blotting as described in the Material and Methods. (B) Exponential growing HMEC control (C) and DNRII cells were harvested and Western blotting was performed as described in the Material and Methods. The phosphorylated MAP kinases are labeled with “p-“, whereas total MAP kinases are labeled with “T-“.