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. Author manuscript; available in PMC: 2008 Mar 1.

Published in final edited form as: Virology. 2006 Oct 18;359(1):19–27. doi: 10.1016/j.virol.2006.09.032

Fig. 4 — Contribution of RBP-Jκ to transactivation of the K8 DE promoter. Wild-type (OT13) and RBP-Jκ-null (OT11) mouse embryonic fibroblast cell lines were cotransfected with 100 ng of K8 promoter-luciferase reporters and 100 ng RTA expression vector (pCR3.1-ORF50 or empty pCR3.1) or 100 ng of RBP-Jκ expression vector or combination of pCR3.1-ORF50 and increasing amounts (0–200 ng) of RBP-Jκ expression vector. (A) Luciferase activities were analyzed by the dual-luciferase reporter assay. Relative luciferase activities were measured as fold activity relative to the basal level of luciferase activity in cells that are cotransfected with blank plasmids, with the error bars representing standard deviations of the results from two independent experiments. (B) Western blotting showing the levels of endogenous RBP-Jκ in OT13 cells, the absence of endogenous RBP-Jκ in OT11 cells and ectopic expression of RBP-Jκ in OT11 cells.

Fig. 4 — Contribution of RBP-Jκ to transactivation of the K8 DE promoter. Wild-type (OT13) and RBP-Jκ-null (OT11) mouse embryonic fibroblast cell lines were cotransfected with 100 ng of K8 promoter-luciferase reporters and 100 ng RTA expression vector (pCR3.1-ORF50 or empty pCR3.1) or 100 ng of RBP-Jκ expression vector or combination of pCR3.1-ORF50 and increasing amounts (0–200 ng) of RBP-Jκ expression vector. (A) Luciferase activities were analyzed by the dual-luciferase reporter assay. Relative luciferase activities were measured as fold activity relative to the basal level of luciferase activity in cells that are cotransfected with blank plasmids, with the error bars representing standard deviations of the results from two independent experiments. (B) Western blotting showing the levels of endogenous RBP-Jκ in OT13 cells, the absence of endogenous RBP-Jκ in OT11 cells and ectopic expression of RBP-Jκ in OT11 cells.