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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Int J Oncol. 2007 Jul;31(1):19–27.

Figure 1.

Figure 1

SNB19 cells were transfected with mock, empty vector (EV)/scrambled vector (SV), puPA, puPAR and pU2 plasmid constructs. After 48 h, cells were collected, total cell lysates were prepared, fractionated by SDS-PAGE, and western blotted as per standard protocols. The blots were immunoprobed for uPAR and GAPDH (A). To determine the enzyme activity of uPA, conditioned media from SNB19 cells transfected with mock, empty vector (EV)/scrambled vector (SV), puPA, puPAR and pU2 were collected and fractionated on a fibrin gel as described earlier (A). Quantitative analysis of uPAR protein levels and uPA enzyme activity per 10×106 cells was normalized to GAPDH expression levels (B). The values shown are mean ±SD from four different experiments (p<0.002)