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SNB19 cells were transfected with mock, empty vector (EV)/scrambled vector (SV), puPA, puPAR and pU2 plasmid constructs. After 48 h, cells were collected, total cell lysates were prepared, fractionated by SDS-PAGE, and western blotted as per standard protocols. The blots were immunoprobed for activated Caspase 8, cleaved PARP, AKT, pAKT, PI3K, mTOR, pmTOR, RAPTOR and Ki67. GAPDH level served as a loading control.
