The effect of pH and NaCl concentration on the resultant incorporation ratio of GTP-TR with α2M* Complexes were prepared following non-proteolytic activation of α2M, by incubation of α2M* with a 40-fold molar excess GTP-TR at 37°C for 18h, in 25mM HEPES buffer solutions of pH 6.5, 7.0, 8.0 and 9.0 with NaCl concentration of 100mM () or 200mM (). All data points are the mean ± 1 SEM, with n=6.