Figure 1.

Delimiting the interaction between E4F and LANP. (A) Interaction of full-length bait LANP with prey E4F constructs (full-length E4F (E4F 1–773) or deletion constructs as indicated, with zinc-finger motifs shown as black boxes). (B) Full-length (FL), amino-terminal (ΔN) and carboxy-terminal (ΔC) terminal deletion constructs of LANP were tested as interacting baits against full-length E4F as prey. Interaction was scored on the basis of relative growth on auxotrophic (4−) selection media (−Trp/−Leu/−His/−Ade); the presence of both bait and prey plasmids is recorded by growth on nonselective (2−) media (−Trp/−Leu). LANP, leucine-rich acidic nuclear protein; LRR, leucine-rich-repeat domain.